Authors: CHOI, Young-whan; KIM, Kyeok; JO, Ji-yeong¹; KIM, Hyo-lim¹; LEE, You-jin²; SHIN, Woo-jung²; SACKET, Santosh J¹; HAN, Mijin¹; IM, Dong-soon

Source: Acta Pharmacologica Sinica, Volume 29, Number 9, September 2008 , pp. 1006-1012(7)

Publisher: Wiley-Blackwell

Abstract:

Aim: To study the effects of dibenzocyclooctadiene lignans isolated from Schisandra chinensis, such as wuweizisu C, gomisin N, gomisin A, and schisandrin, on the membrane potential in C6 glioma cells. Methods: The membrane potential was estimated by measuring the fluorescence change in DiBAC-loaded glioma cells. Results: Wuweizisu C decreased the membrane potential in a concentration-dependent manner. Gomisin N and gomisin A, however, showed differential modulation and no change was induced by schisandrin or dimethyl-4,4′-dimethoxy-5,6,5′,6′-dimethylene dioxybipheny 1-2,2′-dicarboxylate, a synthetic drug derived from dibenzocyclooctadiene lignans. We found no involvement of G_i/o proteins, phospholipase C, and extracellular Na⁺ on the wuweizisu C-induced decrease of the membrane potential. Wuweizisu C by itself did not change the intracellular Ca²⁺ [Ca²⁺]_i concentration, but decreased the ATP-indu-ced Ca²⁺ increase in C6 glioma cells. The 4 lignans at all concentrations used in this study did not induce any effect on cell viability. Furthermore, we found a similar decrease of the membrane potential by wuweizisu C in PC 12 neuronal cells. Conclusion: Our results suggest that the decrease in the membrane potential and the modulation of [Ca²⁺]_i concentration by wuweizisu C could be important action mechanisms of wuweizisu C.

Keywords: Schisandra chinensis; lignan; wuweizisu C; membrane potential; glioma; dimethyl-4,4′-dimethoxy-5,6,5′,6′-dimethylene dioxybiphenyl-2,2′-dicarboxylate

Document Type: Research article

DOI: http://dx.doi.org/10.1111/j.1745-7254.2008.00838.x

Affiliations: 1: Laboratory of Pharmacology, College of Pharmacy (BK21 project) and Longevity Life Science and Technology Institutes, Pusan National University, Busan 609-735, Republic of Korea 2: Department of Horticultural Bioscience Pusan National University, Miryang-si 627-706, Republic of Korea

Publication date: 2008-09-01

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