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Valproic acid-mediated transcriptional regulation of human GM3 synthase (hST3Gal V) in SK-N-BE(2)-C human neuroblastoma cells

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Abstract:

Abstract

Aim: To investigate whether valproic acid (VPA) modulates human GM3 synthase (hST3Gal V) mRNA expression, as a part of ganglioside GM3 biosynthesis, in human neuroblastoma cells. Methods: Using RT-PCR and immunofluo-rescent confocal microscopy, we examined hST3 Gal V mRNA and GM3 levels during VPA-induced differentiation of human neuroblastoma SK-N-BE(2)-C cells. We characterized the VPA-inducible promoter region within the hST3-Gal V gene using luciferase constructs carrying 5′-deletions of the hST3Gal V promoter. Results: RT-PCR indicated that VPA-mediated hST3 Gal V induction is transcriptionally regulated. Functional analysis of the 5′-flanking region of the hST3Gal V gene demonstrated that the -177 to -83 region, which contains a cAMP-responsive element (CRE) at -143, functions as the VPA-inducible promoter by actively binding CRE binding protein (CREB). In addition, site-directed mutagenesis and electrophoretic mobility shift assay indicated that the CRE at -143 is crucial for the VPA-induced expression of hST3Gal V in SK-N-BE(2)-C cells. Conclusion: Our results isolated the core promoter region in the hST3 Gal V promoter, a CRE at -143, and demonstrated that it is essential for transcriptional activation of hST3Gal V in VPA-induced SK-N-BE(2)-C cells. Subsequent CREB binding to this CRE mediates VPA-dependent upregulation of hST3 Gal V gene expression.

Keywords: CREB; human GM3 synthase; human neuroblastoma; transcriptional regulation; valproic acid

Document Type: Research Article

DOI: http://dx.doi.org/10.1111/j.1745-7254.2008.00847.x

Affiliations: 1: Brain Korea 21 Center for Silver-Bio Industrialization, Dong-A University, Busan 604–714, Republic of Korea 2: Molecular and Cellular Glycobiology Unit, Department of Biological Sciences, SungKyunKwan University, Kyunggi-Do 440–746, Republic of Korea; 3: Department of Life Science, Ajou University, Kyunggi-Do 443-749, Republic of Korea

Publication date: September 1, 2008

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