Skip to main content

LY294002 induces p53-dependent apoptosis of SGC7901 gastric cancer cells

Buy Article:

$43.00 plus tax (Refund Policy)

Abstract

Aim: To study the effects of LY294002, an inhibitor of class I phosphatidylinositol 3-kinase (PI3K), on proliferation and apoptosis of SGC7901 gastric cancer cells. Methods: The MTT assay was used to determine the cytotoxic effects of LY294002. Cell cycle distribution was analyzed using flow cytometry and apoptosis was assessed using flow cytometry analysis after staining DNA with propidium iodide. Mitochondrial membrane potential was measured using the fluorescent probe JC-1. Expression of p53 and PUMA was determined using real-time RT-PCR and Western blotting analysis. Results: The viability of SGC7901 cells was significantly reduced by LY294002 treatment. Expression of p53 and PUMA was induced, and mitochondrial membrane potential collapsed after treatment with LY294002. LY294002 induced apoptotic cell death. Conclusion: Activation of the p53 pathway is involved in LY294002-induced SGC7901 cell death.
No References
No Citations
No Supplementary Data
No Data/Media
No Metrics

Keywords: LY294002; cancer; p53

Document Type: Research Article

Affiliations: 1: Department of Neurology, The Second Affiliated Hospital, Soochow University, Suzhou 215004, China 2: Laboratory of Aging and Nervous Diseases, Soochow University School of Medicine, Suzhou 215123, China 3: Department of General Surgery, The Second Affiliated Hospital, Soochow University, Suzhou 215004, China

Publication date: 2008-04-01

  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more