IngentaConnect Gene expression pattern in apoptotic QGY-7703 cells induced by ho...

Authors: JIN, Wei¹; QU, Le-feng²; CHEN, Qin³; CHANG, Xin-zhong¹; WU, Jiong¹; SHAO, Zhi-min

Source: Acta Pharmacologica Sinica, Volume 28, Number 6, June 2007 , pp. 859-868(10)

Abstract:

Aim: To classify the genes responsible for apoptosis in QGY-7703 cells induced by homoharringtonine (HHT). Methods: Apoptosis in QGY-7703 cells induced by HHT was demonstrated by DNA fragmentation and morphological observation. cDNA microarray technology was used to detect gene transcription, and the result of microarrays for genes was confirmed by RT-PCR. Results: Seventy-eight individual mRNA were identified and their transcription levels changed significantly. Those genes, of which 68% were upregulated and 32% were downregulated, were partially related to apoptosis. They were mostly oncogenes, tumor suppressors, enzymes, and kinases. Conclusion: HHT is a potential drug in the treatment of liver cancer. TGF-β, TNF, FAS, p38 MAP K, and p53 apoptosis signaling pathways were activated during apoptosis in QGY-7703 cells. Such inducible genes may play important roles in apoptosis and deserve to be further studied.

Keywords: QGY-7703 cells; homoharringtonine; apoptosis; gene expression

Document Type: Research article

DOI: 10.1111/j.1745-7254.2007.00569.x

Affiliations: 1: Breast Cancer Institute, Cancer Hospital, Department of Oncology, Shanghai Medical College, Institute of Biomedical Science, Fudan University, Shanghai 200032, China 2: Department of Vascular Surgery and Institute of Vascular Surgery of PLA, Changhai Hospital, Shanghai 200433, China 3: Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China

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Gene expression pattern in apoptotic QGY-7703 cells induced by homoharringtonine