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Efflux of potassium ion is an important reason of HL-60 cells apoptosis induced by tachyplesin

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Abstract:

Abstract

Aim: To investigate the role of intercellular potassium in tachyplesin-induced HL-60 cells apoptosis. Methods: The concentration of intercellular potassium, cell volume and mitochondrial membrane potential were examined by flow cytometry. Results: The concentration of intercellular potassium reduced in a time-dependent manner in tachyplesin-treated HL-60 cells. In addition, the loss of mitochondrial membrane potential was tightly coupled with the shrinkage of cell volume. Different caspase inhibitors protected against DNA degradation but did not prevent the loss of HL-60 cell viability induced by tachyplesin. Ba2+, which was a kind of blocker of volume-regulatory K+ channels, increased the viability of tachyplesin-treated HL-60 cells and maintained mitochondrial membrane potential and cell volume. Conclusion: Efflux of K+ was an important reason for apoptosis in tachyplesin-treated HL-60 cells. Efflux of K+ affected the viability of tachyplesin-treated HL-60 cells independent of the process of caspase activation.

Keywords: HL-60 cell; horseshoe crab; intracellular potassium; mitochondrial membrane potential; tachyplesin

Document Type: Research Article

DOI: https://doi.org/10.1111/j.1745-7254.2006.00377.x

Affiliations: 1: Affiliated Hospital of Guangdong Medical College, Zhanjiang 524023, China 2: Institute of Biochemistry and Molecular Biology, Guangdong Medical College, Zhanjiang 524023, China

Publication date: 2006-10-01

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