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Calcitonin gene-related peptide inhibits interleukin-1 -induced interleukin-8 secretion in human type II alveolar epithelial cells

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Abstract:

Abstract

Aim: Our previous data have shown that type II alveolar epithelial (AEII) cells express neuropeptide calcitonin gene-related peptide (CGRP), and that pro-inflammatory factor interleukin1- (IL-1) induces CGRP secretion in the A549 human AEII cell line. In the present study, we investigated the effect of endogenous and exogenous CGRP on IL-1-induced chemokine interleukin-8 (IL-8) secretion. Methods: We used enzyme-linked immunosorbent assay (ELISA) and RT-PCRto detect IL-8 protein and mRNA levels, respectively. siRNA and the stably trans-fected cell line were used to knock down and overexpress the CGRP gene, respectively, and chemiluminescence assay was used to detect reactive oxygen species (ROS) formation. Results: CGRP-1 receptor antagonist hCGRP8–37 (0.1–1 nmol-L−1) greatly amplified IL-1 -induced IL-8 production. The inhibition of CGRP expression by siRNA significantly increased IL-8 secretion upon IL-1 stimulation. However, cell clones stably transfected with CGRP showed significantly inhibited mRNAandproteinlevelsofIL-8 inducedbyIL-1. Conclusion: These data imply that AEII cell-derived CGRP suppress IL-1 -induced IL-8 secretion in an autocrine/paracrine mode. Further investigation showed that CGRP attenuated IL-1 -aroused ROS formation, which is an early indication of pro-inflammatory factor signaling.

Keywords: anti-inflammation; calcitonin gene-related peptide; interleukin-1; interleukin-8; lung; reactive oxygen species; type II alveolar epithelial cells

Document Type: Research Article

DOI: http://dx.doi.org/10.1111/j.1745-7254.2006.00408.x

Affiliations: Department of Physiology, School of Basic Medical Sciences, Peking University, Key Laboratory of Molecular Cardiovascular Science, Ministry of Education, Beijing 100083, China

Publication date: October 1, 2006

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