Authors: LI, Yi-tang¹; YAO, Chun-suo²; BAI, Jin-ye¹; LIN, Mao²; CHENG, Gui-fang

Source: Acta Pharmacologica Sinica, Volume 27, Number 6, June 2006 , pp. 735-740(6)

Publisher: Wiley-Blackwell

Abstract:

Aim: To explore the anti-inflammatory effects of amurensin H on asthma-like reaction induced by allergen in sensitized mice. Methods: BALB/c mice were sensitized by ovalbumin (OVA, ip) on d 0 and d 14 and challenged with 1 % OVA on d 18 to 22. Mice developed airway eosinophilia, mucus hypersecretion, and elevation in cytokine levels. Mice were administered amurensin H orally at the doses of 49, 70, or 100 mg/kg once every day from d 15 to the last day. Bronchoalveolar lavage fluid (BALF) were collected at 24 h and 48 h after the last OVA challenge. Levels of tumor necrosis factor-α (TNF-α), interleukin 4 (IL-4), interleukin 5 (IL-5), and interleukin 13 (IL-13) in BALF were measured using ELISA method. Differential cell counts of macrophages, lymphocytes, neutrophils and eosinophils were performed in 200 cells per slide (one slide per animal). Lung tissue sections of 6-um thickness were stained with Mayer's hematoxylin and eosin for assessment of cell infiltration, mucus production, and tissue damage. Results: Oral administration of amurensin H significantly inhibited OVA-induced increases in total cell counts, eosinophil counts, and TNF-α, IL-4, IL-5 and IL-13 levels in BALF. In addition, amuresin H dramatically decreased OVA-induced lung tissue damage and mucus production. Conclusion: Amurensin H may have therapeutic potential for the treatment of allergic airway inflammation.

Keywords: amurensin H; asthma; ovalbumin; tumor necrosis factor-α; Th2 cells; cytokines

Document Type: Research article

DOI: http://dx.doi.org/10.1111/j.1745-7254.2006.00330.x

Affiliations: 1: Department of Pharmacology, Institute of Materia Medica, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100050, China 2: Department of Photochemistry, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China

Publication date: 2006-06-01

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