Soybean isoflavones alter parvalbumin in hippocampus of mid-aged normal female, ovariectomized female, and normal male rats

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Abstract:

Abstract

Aim: To investigate the long-term effect of soybean isoflavones on changes in parvalbumin (P V) immunoreactivity in the hippocampus in normal female, ovariectomized (OVX) female and normal male rats. Methods: Ten-month-old rats were assigned to one of 9 groups (n=7 in each group) based on body weight using a randomized complete-block design. The groups were: control diet-treated females, OVX females, and males; 0.3 g/kg isoflavone-treated females, OVX females, and males; and 1.2 g/kg isoflavone-treated females, OVX females, and males. The PV immunostaining was conducted by using the standard avidin-biotin complex method. Results: PV immunoreactivity and the number of PV-immunoreactive neurons in all the groups after isoflavone treatment were significantly changed in the hippocampal CA1 region and in the dentate gyrus, but not in the hippocampal CA2/3 region. PV immunoreactivity and the number of PV-immunoreactive neurons in the control diet OVX females were similar to those in the control diet, and were greater than those in the control diet normal females. PV immunoreactivity and the number of PV-immunoreactive neurons in all the isoflavone-treated groups decreased dose-dependently after isoflavone treatment. Conclusion: Long-term administration of isoflavones may induce a reduction of PV in interneurons in the hippocampal CA1 region and in the dentate gyrus. The reduction of PV in these regions suggests that the long-term administration of isoflavones may cause a change in calcium homeostasis in the hippocampal CA1 region and in the dentate gyrus.

Keywords: hippocampus; immunohistochemistry; isoflavones; ovariectomy

Document Type: Research Article

DOI: http://dx.doi.org/10.1111/j.1745-7254.2006.00258.x

Affiliations: 1: Department of Anatomy, College of Medicine, Hallym University, Chunchon 200-702, Korea 2: Department of Laboratory Animal Research, Central Research Institute, Dr Chung's Food Co Ltd, Cheongju 361-782, Korea 3: Division of Life Science and Silver Biotechnology Research Center, Hallym University, Chunchon 200-702, Korea 4: Faculty of Marine Bioscience and Technology, Kangneung National University, Kangneung 210-702, Korea 5: Faculty of Food Science and Technology, College of Life Sciences, Sejong University, Sejong 143-747, Korea

Publication date: January 1, 2006

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