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Assessing phylogenetic relationships of Lycium samples using RAPD and entropy theory

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Abstract Aim:

To evaluate the phylogenetic relationships among related species of Lycium samples. Methods:

Random amplified polymorphic DNA (RAPD) fingerprinting and lab-on-a-chip electrophoresis techniques were used to analyze the characteristics of Lycium species. Seven species and 3 varieties of Lycium were studied. Based on RAPD fingerprint data obtained from 11 primers, we proposed a new index, called dispersivity, using entropy theory and projection methods to depict the diversity of the DNA fingerprints. Results:

Using the proposed dispersivity, primers were sorted and the dendrograms of the 7 species and 3 varieties of Lycium were constructed synthetically by merging primer information. Conclusion:

Phylogenetic relationships among Lycium samples were constructed synthetically based on RAPD fingerprint data generated from 11 primers.

Keywords: DNA fingerprinting; RAPD technique; UPGMA; entropy; lab-on-a-chip

Document Type: Research Article

DOI: http://dx.doi.org/10.1111/j.1745-7254.2005.00197.x

Affiliations: 1: School of Mathematics and Statistics, Northeast Normal University, Changchun 130024, China; 2: Department of Mathematics, Hong Kong Baptist University, Hong Kong, China; 3: College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China; 4: Department of Biology, Hong Kong Baptist University, Hong Kong, China

Publication date: October 1, 2005

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