Skip to main content

Effects of propyl gallate on interaction between TNF-α and sTNFR-I using an affinity biosensor

Buy Article:

$51.00 plus tax (Refund Policy)

Abstract:

Abstract Aim:

To study the effects of propyl gallate on the interaction of tumor necrosis factor-α (TNF-α) with its soluble receptor, sTNFR-I. Methods:

Interactions between TNF-α and sTNFR-I were analyzed using an IAsys biosensor. sTNFR-I was immobilized on the carboxymethyl dextran (CMD) surface of the IAsys biosensor cuvettes, and TNF-α preincubated with different concentrations of propyl gallate was added to the cuvettes. The resonant angle shift caused by the binding between TNF-α and sTNFR-I was then recorded. Results:

sTNFR-I was immobilized on the CMD surface at a density of 2.76 ng/mm2. TNF-α then bound the immobilized sTNFR-I specifically, and propyl gallate was able to enhance the binding between TNF-α and sTNFR-I in a dose-dependent manner. Conclusion:

The binding between TNF-α and sTNFR-I is one of the targets that propyl gallate can act on in vivo. The IAsys biosensor offers a new clue as to the study on the mechanisms of action of propyl gallate.

Keywords: affinity biosensor; propyl gallate; soluble tumor necrosis factor receptor; tumor necrosis factor-α

Document Type: Research Article

DOI: https://doi.org/10.1111/j.1745-7254.2005.00198.x

Affiliations: Laboratory of Biosensors, College of Life Science, Nankai University, Tianjin 300071, China

Publication date: 2005-10-01

  • Access Key
  • Free ContentFree content
  • Partial Free ContentPartial Free content
  • New ContentNew content
  • Open Access ContentOpen access content
  • Partial Open Access ContentPartial Open access content
  • Subscribed ContentSubscribed content
  • Partial Subscribed ContentPartial Subscribed content
  • Free Trial ContentFree trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more