Effects of propyl gallate on interaction between TNF-α and sTNFR-I using an affinity biosensor

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Abstract Aim:

To study the effects of propyl gallate on the interaction of tumor necrosis factor-α (TNF-α) with its soluble receptor, sTNFR-I. Methods:

Interactions between TNF-α and sTNFR-I were analyzed using an IAsys biosensor. sTNFR-I was immobilized on the carboxymethyl dextran (CMD) surface of the IAsys biosensor cuvettes, and TNF-α preincubated with different concentrations of propyl gallate was added to the cuvettes. The resonant angle shift caused by the binding between TNF-α and sTNFR-I was then recorded. Results:

sTNFR-I was immobilized on the CMD surface at a density of 2.76 ng/mm2. TNF-α then bound the immobilized sTNFR-I specifically, and propyl gallate was able to enhance the binding between TNF-α and sTNFR-I in a dose-dependent manner. Conclusion:

The binding between TNF-α and sTNFR-I is one of the targets that propyl gallate can act on in vivo. The IAsys biosensor offers a new clue as to the study on the mechanisms of action of propyl gallate.

Keywords: affinity biosensor; propyl gallate; soluble tumor necrosis factor receptor; tumor necrosis factor-α

Document Type: Research Article

DOI: http://dx.doi.org/10.1111/j.1745-7254.2005.00198.x

Affiliations: Laboratory of Biosensors, College of Life Science, Nankai University, Tianjin 300071, China

Publication date: October 1, 2005

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