Skip to main content

Novel cyclophilin D inhibitors derived from quinoxaline exhibit highly inhibitory activity against rat mitochondrial swelling and Ca2+ uptake/release

Buy Article:

$43.00 plus tax (Refund Policy)

Abstract Aim:

To investigate methods for identifying specific cyclophilin D (CypD) inhibitors derived from quinoxaline, thus developing possible lead compounds to inhibit mitochondrial permeability transition (MPT) pore opening. Methods:

Kinetic analysis of the CypD/inhibitor interaction was quantitatively performed by using surface plasmon resonance (SPR) and fluorescence titration (FT) techniques. IC50 values of these inhibitors were determined by PPIase inhibition activity assays. Results:

All the equilibrium dissociation constants (KD) of the seven compounds binding to CypD were below 10 mol/L. The IC50 values were all consistent with the SPR and FT results. Compounds GW2, 5, 6, and 7 had high inhibition activities against Ca2+-dependent rat liver mitochondrial swelling and Ca2+ uptake/release. Compound GW5 had binding selectivity for CypD over CypA. Conclusion:

The agreement between the measured IC50 values and the results of SPR and FT suggests that these methods are appropriate and powerful methods for identifying CypD inhibitors. The compounds we screened using these methods (GW1-7) are reasonable CypD inhibitors. Its potent ability to inhibit mitochondrial swelling and the binding selectivity of GW5 indicates that GW5 could potentially be used for inhibiting MPT pore opening.
No References
No Citations
No Supplementary Data
No Data/Media
No Metrics

Keywords: cyclophilin; fluorescence titration; inhibitor; mitochondrial permeability transition; quinoxalines; surface plasmon resonance

Document Type: Research Article

Publication date: 2005-10-01

  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more