The effect of indole-3-carbinol and sulforaphane on a prostate cancer cell line
Cruciferous vegetable consumption is inversely related to the incidence of prostate cancer. We examined the effect of indole-3-carbinol (I3C) and of sulforaphane (constituents of cruciferous vegetables) on cell proliferation of a PC-3 prostate cancer cell line, in order to observe if an inhibitory effect might be detected in vitro. Methods:
PC-3 prostate cancer cells were cultured in 96-well microtitre plates. Indole-3-carbinol concentrations ranging from 0.1 mmol/L to 0.8 mmol/L or sulforaphane concentrations ranging from 0.01 mmol/L to 0.06 mmol/L were added to the wells. Cell proliferation was measured by colorimetric assay and results were based on the mean value of triplicate experiments. Data are presented as medians and interquartile ranges and were analysed using the Mann−Whitney U-test. Results:
Cell proliferation in PC-3 prostate cancer cells was significantly inhibited by I3C and sulforaphane at media concentrations of 0.2 mmol/L and 0.02 mmol/L, respectively. Conclusion:
Both compounds inhibited the proliferation of prostate cancer cells in a dose-dependant manner. These findings may help explain the observed protective effect of cruciferous vegetables in relation to prostate cancer.
Document Type: Research Article
Affiliations: Discipline of Surgical Science, Faculty of Health, The University of Newcastle, New South Wales, Australia
Publication date: March 1, 2003