Oral phosphodiesterase-5 inhibitors: effect of heme oxygenase inhibition on cGMP signalling in rat cavernous tissue

Authors: Abdel Aziz, M. T.1; Mostafa, T.2; Atta, H.1; Rashed, L.1; Marzouk, S. A.1; Obaia, E. M.1; Sabry, D.1; Hassouna, A. A.1; El-Shehaby, A. M.1; Abdel Aziz, A.T.1

Source: Andrologia, Volume 39, Number 2, April 2007 , pp. 66-70(5)

Publisher: Blackwell Publishing

Key:
Free Content - Free Content
New Content - New Content
Subscribed Content - Subscribed Content
Free Trial Content - Free Trial Content

Abstract:

Summary

This work postulated that heme oxygenase (HO) is partly responsible for controlling phosphodiesterase-5 inhibitor actions by modulating cyclic guanosine monophosphate (cGMP) cavernous tissue levels. Five hundred and four male Sprague-Dawley rats, divided into five groups, were investigated. Group 1 (n = 72) included controls, group 2 (n = 72) received sildenafil citrate (ViagraR) orally, group 3 (n = 72) received vardenafil hydrochloride (LevitraR), group 4 (n = 72) received tadalafil (CialisR). Group 5 (n = 216), subdivided into three subgroups (A, B and C, 72 each), received the same dose of each drug with the HO inhibitor, Zn protoporphyrin. Eight rats from each group/subgroup were killed at 0.5, 1, 2, 3, 4, 6, 18, 24 and 36 h when cGMP levels in the cavernous tissues were estimated. Cavernous tissue cGMP levels increased significantly in sildenafil, vardenafil and tadalafil-treated rats compared to the controls with significant decreases after HO inhibition. It is concluded that the effects of these PDE-5 inhibitors in rat cavernous tissue are partly mediated through HO activity via the cGMP signalling pathway.

Keywords: cGMP; erectile dysfunction; heme oxygenase; PDE inhibitors; sildenafil; tadalafil; vardenafil

Document Type: Research article

DOI: 10.1111/j.1439-0272.2007.00765.x

Affiliations: 1: Molecular Biology Unit, Department of Medical Biochemistry, Faculty of Medicine, Cairo University, Cairo, Egypt; 2: Department of Andrology & Sexology, Faculty of Medicine, Cairo University, Cairo, Egypt

The full text electronic article is available for purchase. You will be able to download the full text electronic article after payment.

$50.39 plus tax      Refund Policy

 

OR

Back to top

Key:
Free Content - Free Content
New Content - New Content
Subscribed Content - Subscribed Content
Free Trial Content - Free Trial Content
Share this item with others: These icons link to social bookmarking sites where readers can share and discover new web pages.
Page Help Click here for Page Help
Shopping cart
Tools
Sign in






Need to register?
Sign up here
Text size: A | A | A | A