Improvement in erectile dysfunction after insulin-like growth factor-1 gene therapy in diabetic rats

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Abstract:

Abstract

Aim: To determine whether adenoviral gene transfer of insulin like growth factor-1 (IGF-1) to the penis of streptozotocin (STZ)-induced diabetic rats could improve erectile capacity. Methods: The STZ diabetic rats were transfected with AdCMV-gal or AdCMV-IGF-1. These rats underwent cavernous nerve stimulation to assess erectile function and their responses were compared with those of age-matched control rats 1 to 2 days after transfection. In control and transfected STZ diabetic rats, IGF-1 expression were examined by reverse transcription polymerase chain reaction (RT-PCR), Western blot and histology. The penis -galactosidase activity and localization of the STZ diabetic rats were also determined. Results: One to two days after transfection, the -galactosidase was found in the smooth muscle cells of the diabetic rat penis transfected with AdCMV-gal. One to 2 days after administration of AdCMV-IGF-1, the cavernosal pressure, as determined by the ratio of maximal intracavernous pressure-to-mean arterial pressure (ICP/MAP) and total intracavernous pressure (ICP), was increased in response to cavernous nerve stimulation. Transgene expression was confirmed by RT-PCR, Western blot and histology. Conclusion: Gene transfer of IGF-1 significantly increased erectile function in the STZ diabetic rats. These results suggest that in vivo gene transfer of IGF-1 might be a new therapeutic intervention for the treatment of erectile dysfunction (ED) in the STZ diabetic rats.

Edited by Prof. Aleksander Giwercman

Keywords: cavernosometry; erectile dysfunction; gene therapy; insulin like growth factor-1

Document Type: Original Article

DOI: http://dx.doi.org/10.1111/j.1745-7262.2007.00215.x

Affiliations: 1: Department of Urology, Guangdong Provicial People's Hospital, Guangzhou 510080, China 2: Departments of Andrology and Urology, Zhongnan Hospital, Wuhan University, Wuhan 430071, China

Publication date: January 1, 2007

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