Authors: Persmark, Lotta¹; Banck, Anita²; Andersson, Stig²; Jansson, Hans-Börje¹

Source: Nematologica, Volume 38, Numbers 1-4, 1992 , pp. 520-530(11)

Publisher: BRILL

Abstract:

Six methods were tested for nematode extraction: the Seinhorst elutriator, Cobb's decanting and sieving, the Whitehead direct method (Whitehead tray), and centrifugal-flotation with silica (Ludox LS), sucrose and MgSO₄, respectively. The most efficient methods for non-parasitic nematodes were centrifugal-flotation with silica, the Seinhorst elutriator and the Whitehead tray. Tylenchorhynchus spp. and total plant parasitic nematodes were recovered in largest numbers with the elutriator and the three centrifugation-flotation methods. The largest numbers of Pratylenchus crenatus were recovered with the Whitehead tray, while centrifugal-flotation with silica was superior for the trichodorids. Four species of endoparasitic nematophagous fungi (Catenaria anguillulae, Harposporium anguillulae, Nematoctonus leiosporus and Hirsutella rhossiliensis) were isolated from nematodes extracted with four of the six methods: the Seinhorst elutriator, the Whitehead tray, centrifugation with silica and centrifugation with sucrose. The largest numbers of infected nematodes were obtained with centrifugal-flotation with silica and the elutriation method. The smallest number of endoparasitic fungi were recovered by using Cobb's decanting and sieving and the Whitehead tray methods.

Keywords: Catenaria anguillulae; Harposporium anguillulae; Nematoctonus leiosporus; Hirsutella rhossiliensis; Tylenchorhynchus spp; Pratylenchus crenatus; Trichodorus viruliferus; Paratrichodorus pachydermus; extraction techniques

Document Type: Research article

DOI: http://dx.doi.org/10.1163/187529292X00496

Affiliations: 1: Department of Microbial Ecology, Lund University, Ecology Building, Helgonavägen 5, S-223 62 Lund 2: Department of Plant and Forest Protection, Swedish University of Agricultural Sciences, Box 44, S-230 53 Alnarp, Sweden

Publication date: 1992-01-01

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