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Estimating the Concentration of Propagules of a Plant Pathogen from Soil Dilution Plate Counts when the Response is Non-linear

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The estimation of micro-organism concentrations from dilution plate data is discussed for situations where expected counts are not proportional to the amount of sample per plate. Aspects of design and analysis are investigated in relation to an alternative non-linear model in which the concentration is given by the slope at the origin. This exponential model generally provides a good fit to available experimental data. Simulations show that estimators based on the model perform well when the response is non-linear and remain reasonably efficient when the response is linear.

Keywords: Dilution series; Exponential model; Maximum likelihood estimation; Optimal design; Pathogenic fungi; Plate count method

Document Type: Original Article


Affiliations: Horticulture Research International, East Malling, UK

Publication date: January 1, 1997


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