Authors: PEARSON, LAURA; THOM, JIM¹; ADAMS, MURRAY²; OOSTRYCK, ROBERT²; KRUEGER, ROM¹; YONG, GERALD³; BAKER, ROSS

Source: International Journal of Laboratory Hematology, Volume 31, Number 4, August 2009 , pp. 430-439(10)

Publisher: Wiley-Blackwell

Abstract:

Summary

Platelet activation occurs in a variety of clinical situations in which it directly contributes to the pathology. This study reports a simple flow cytometric assay for platelet activation which measures platelet-derived microparticles, activated platelets and platelet-monocyte complexes. Pre- and post analytical conditions were investigated and optimized and a normal range established on 20 healthy controls. Twenty patients pre- and post percutaneous coronary intervention (PCI) were tested with the technique. Soluble activation markers sCD40 ligand and sP-selectin and plasma phospholipid levels were measured in both groups. There was a significant increase in activated platelets and platelet-monocyte complexes between normal and pre-PCI (P = 0.005 and 0.0275, respectively) suggesting an activated state. There was a significant fall in activated platelets post-PCI (P = 0.0027) which was mirrored by a fall in soluble CD40 ligand, soluble P-selectin and plasma phospholipid levels (P = 0.0066, <0.0001 and 0.0032, respectively) consistent with antiplatelet therapy administered during the process. This is a reliable and rapid method for the assessment of ex vivo platelet activation which may be an aid in diagnosis and help guide therapy for patients with thrombotic disease.

Keywords: Activated platelets; flow cytometry; percutaneous coronary intervention; platelet-monocyte complexes

Document Type: Research article

DOI: http://dx.doi.org/10.1111/j.1751-553X.2008.01059.x

Affiliations: 1: Haematology Department, Royal Perth Hospital, Perth, Australia 2: School of Biomedical Sciences, Curtin University, Perth, Australia 3: Cardiology Department, Royal Perth Hospital, Perth, Australia

Publication date: 2009-08-01

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• In this Subject: Anatomy & Physiology ,  Biology ,  Medicine (General)
• By this author: PEARSON, LAURA ;  THOM, JIM ;  ADAMS, MURRAY ;  OOSTRYCK, ROBERT ;  KRUEGER, ROM ;  YONG, GERALD ;  BAKER, ROSS

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