Expression, purification, crystallization and preliminary X‐ray analysis of ribitol‐5‐phosphate cytidylyltransferase from
TarI is a ribitol‐5‐phosphate cytidylyltransferase that catalyzes the formation of CDP‐ribitol, which is involved in the biosynthesis of wall teichoic acids, from CTP and ribitol 5‐phosphate. TarI from Bacillus subtilis (BsTarI) was purified and crystallized using the sitting‐drop vapour‐diffusion method. The crystals diffracted to a resolution of 1.78 Å and belonged to the monoclinic space group C2, with unit‐cell parameters a = 103.74, b = 60.97, c = 91.80 Å, β = 113.48°. The initial structural model indicated that the crystals of BsTarI contained a dimer in the asymmetric unit.
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Document Type: Research Article
Publication date: 2012-10-01