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Crystallization and preliminary crystallographic studies of CbsA, a secretory exoglucanase from

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Abstract:

The bacterial pathogen Xanthomonas oryzae pv. oryzae causes bacterial leaf blight, a serious disease of rice. The secreted exoglucanase CbsA is an important virulence factor of this pathogen. It belongs to the glycosyl hydrolase 6 family of proteins based on the carbohydrate‐active enzyme (CAZY) classification. In this study, CbsA has been overexpressed, purified and crystallized. The crystal diffracted to a resolution of 1.86 Å and belonged to space group P212121. It contained one monomer per asymmetric unit, with a solvent content of 45.8%.

Document Type: Research Article

DOI: http://dx.doi.org/10.1107/S1744309112034197

Affiliations: Structural Biology Laboratory, CSIR – Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad 500 007, India

Publication date: October 1, 2012

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