Skip to main content

On‐column ligand exchange for structure‐based drug design: a case study with human 11β‐hydroxysteroid dehydrogenase type 1

Buy Article:

$51.00 plus tax (Refund Policy)

Abstract:

Successfully forming ligand–protein complexes with specific compounds can be¬†a¬†significant challenge in supporting structure‐based drug design for a given protein target. In this respect, an on‐column ligand‐ and detergent‐exchange method was developed to obtain ligand–protein complexes of an adamantane series of compounds with 11β‐hydroxysteroid dehydrogenase type 1 (11β‐HSD1) after a variety of other complexation methods had failed. This report describes the on‐column exchange method and an unexpected byproduct of the method in which artificial trimers were observed in the structures.

Document Type: Research Article

DOI: https://doi.org/10.1107/S1744309112010172

Publication date: 2012-05-01

  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more