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Expression, purification and crystallization of the Crimean–Congo haemorrhagic fever virus nucleocapsid protein

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Abstract:

Crimean–Congo haemorrhagic fever virus (CCHFV) is a member of the Nairovirus genus within the Bunyaviridae family of segmented negative‐sense RNA viruses. This paper describes the expression, purification and crystallization of full‐length CCHFV nucleocapsid (N) protein and the collection of a 2.1 Å resolution X‐ray diffraction data set using synchrotron radiation. Crystals of the CCHFV N protein belonged to space group C2, with unit‐cell parameters a = 150.38, b = 72.06, c = 101.23 Å, β = 110.70° and two molecules in the asymmetric unit. Circular‐dichroism analysis provided insight into the secondary structure, whilst gel‐filtration analysis revealed possible oligomeric states of the N protein. Structural determination is ongoing.

Document Type: Research Article

DOI: http://dx.doi.org/10.1107/S1744309112009736

Affiliations: Institute of Molecular and Cellular Biology, Faculty of Biological Sciences, and Astbury Centre for Molecular and Structural Biology, University of Leeds, Leeds LS2 9JT, England

Publication date: May 1, 2012

bpl/ayf2/2012/00000068/00000005/art00014
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