Expression, purification and crystallization of the Crimean-Congo haemorrhagic fever virus nucleocapsid protein

Authors: Carter, S. D.; Barr, J. N.; Edwards, T. A.

Source: Acta Crystallographica Section F, Volume 68, Number 5, 1 May 2012 , pp. 569-573(5)

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Abstract:

Crimean-Congo haemorrhagic fever virus (CCHFV) is a member of the Nairovirus genus within the Bunyaviridae family of segmented negative-sense RNA viruses. This paper describes the expression, purification and crystallization of full-length CCHFV nucleocapsid (N) protein and the collection of a 2.1 Å resolution X-ray diffraction data set using synchrotron radiation. Crystals of the CCHFV N protein belonged to space group C2, with unit-cell parameters a = 150.38, b = 72.06, c = 101.23 Å, β = 110.70° and two molecules in the asymmetric unit. Circular-dichroism analysis provided insight into the secondary structure, whilst gel-filtration analysis revealed possible oligomeric states of the N protein. Structural determination is ongoing.

Document Type: Research article

DOI: http://dx.doi.org/10.1107/S1744309112009736

Affiliations: 1: Institute of Molecular and Cellular Biology, Faculty of Biological Sciences, and Astbury Centre for Molecular and Structural Biology, University of Leeds, Leeds LS2 9JT, England

Publication date: 2012-05-01