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Crystallization and preliminary X‐ray diffraction analysis of the SOD‐TAT fusion protein

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Abstract:

The superoxide dismutase (SOD) family of proteins are necessary to protect oxygen‐utilizing cells from the toxicity of reactive oxygen species. The delivery of SOD into tissues is severely limited by its size and biochemical properties. A cell‐membrane‐permeable SOD, SOD‐TAT, has been demonstrated to have the ability to be directly transduced into mammalian cells. In this study, the SOD‐TAT fusion protein was expressed, purified and crystallized. Crystals of the SOD‐TAT fusion protein diffracted to 3.20 Å resolution and belonged to space group C121.

Document Type: Research Article

DOI: http://dx.doi.org/10.1107/S1744309112012031

Publication date: May 1, 2012

bpl/ayf2/2012/00000068/00000005/art00008
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