Skip to main content

Expression, purification, crystallization and preliminary X‐ray analysis of carbonyl reductase S1 from

Buy Article:

$51.00 plus tax (Refund Policy)

The NADPH‐dependent carbonyl reductase S1 from Candida magnoliae stereoselectively catalyzes the reduction of ethyl 4‐chloro‐3‐oxobutanoate (COBE) to ethyl (S)‐4‐chloro‐3‐hydroxybutanoate (CHBE), which is a chiral compound valuable as a building block for pharmaceuticals. Carbonyl reductase S1 was expressed in Escherichia coli and purified by Ni‐affinity, ion‐exchange and size‐exclusion chromatography. Crystals of carbonyl reductase S1 were obtained by the sitting‐drop vapour‐diffusion method using PEG 400 as a precipitant. X‐ray diffraction data were collected to 1.90 Å resolution using a synchrotron‐radiation source. The crystals belonged to space group P6122 or P6522, with unit‐cell parameters a = b = 77.7, c = 307.5 Å. The asymmetric unit contained two molecules of the protein, with a solvent content of 44.2%.
No References
No Citations
No Supplementary Data
No Data/Media
No Metrics

Document Type: Research Article

Publication date: 2012-05-01

  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more