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, a cause of urinary tract infections in children?

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Aim:  To inform that Actinobaculum schaalii can colonize the urine and cause urinary tract infection in children.

Methods:  Urine samples were examined by wet smear microscopy, incubated in 5% CO2 for 1–2 days, and species‐specific real‐time polymerase chain reaction (PCR) for A. schaalii was performed.

Results:  In 5 of the 29 screened urines, A. schaalii was found only by real‐time PCR in quantities equivalent to ≥104–105 CFU/mL. In addition, A. schaalii was found in quantities equivalent to ≥106 CFU/mL by both culture and PCR in two children with a urinary tract infection and large numbers of leucocytes in the urine.

Conclusion:  Actinobaculum schaalii is CO2‐dependent. Therefore, if there are clinical symptoms and/or a negative culture despite the presence of leucocytes in the urine, Gram staining and incubation in 5% CO2 or species‐specific real‐time PCR should be performed to identify A. schaalii.

Document Type: Research Article


Affiliations: 1: .Department of Paediatrics, Viborg Regional Hospital, Viborg, Denmark 2: .Department of Clinical Microbiology, Viborg Regional Hospital, Viborg, Denmark

Publication date: May 1, 2012


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