, a cause of urinary tract infections in children?
Aim: To inform that Actinobaculum schaalii can colonize the urine and cause urinary tract infection in children.
Methods: Urine samples were examined by wet smear microscopy, incubated in 5% CO2 for 1–2 days, and species‐specific real‐time polymerase chain reaction (PCR) for A. schaalii was performed.
Results: In 5 of the 29 screened urines, A. schaalii was found only by real‐time PCR in quantities equivalent to ≥104–105 CFU/mL. In addition, A. schaalii was found in quantities equivalent to ≥106 CFU/mL by both culture and PCR in two children with a urinary tract infection and large numbers of leucocytes in the urine.
Conclusion: Actinobaculum schaalii is CO2‐dependent. Therefore, if there are clinical symptoms and/or a negative culture despite the presence of leucocytes in the urine, Gram staining and incubation in 5% CO2 or species‐specific real‐time PCR should be performed to identify A. schaalii.
Document Type: Research Article
Publication date: May 1, 2012