If you are experiencing problems downloading PDF or HTML fulltext, our helpdesk recommend clearing your browser cache and trying again. If you need help in clearing your cache, please click here . Still need help? Email help@ingentaconnect.com

New growth hormone assays: potential benefits

$48.00 plus tax (Refund Policy)

Download / Buy Article:

Abstract:

Three recently published new assays are described for the measurement of growth hormone (GH). Two of these – the eluted stain assay (ESTA) and the immunofunctional assay (IFA) – have been developed to measure the bioactivity of GH, rather than the immunoactivity as measured by conventional radioimmunoassays (RIAs). The third assay – the 22 kDa exclusion assay (22 k GHEA) – is designed to measure the concentrations of the different isoforms of GH present in the circulation. The ESTA is a variant of the Nb2 bioassay for lactogenic hormones, but has been adapted for specific GH measurement in serum samples. It has a lower detection limit than previous bioassays and permits the quantification of GH in large series of samples. The IFA uses a binding‐site‐specific antibody in combination with GH‐binding protein (GHBP) in order to quantify only those GH molecules that are able to dimerize the extracellular domain of the GH receptor (GHBP), which is a prerequisite for GH signal transduction in target cells. The IFA is as convenient to use as immunoassays and can be employed routinely for GH determinations. The clinical usefulness of the 22 k GHEA has not been established, but it should provide a means of augmenting our understanding of the regulation of GH and its various isoforms. Once the ESTA bioassay or the IFA become commercially and widely available, either could replace the RIA as the standard reference method for measuring GH, as both more closely reflect the biologically active proportion of GH in serum samples than that measured by RIA. □ Growth hormone assays, eluted stain assay, immunofunctional assay, bioassay, 22 kDa growth hormone exclusion assay, growth hormone isoforms

Document Type: Review Article

DOI: http://dx.doi.org/10.1111/j.1651-2227.1997.tb18361.x

Affiliations: London Centre for Paediatric Endocrinology & Metabolism, Great Ormond Street Hospital for Children and UCL Hospitals, London, UK

Publication date: November 1, 1997

Related content

Tools

Favourites

Share Content

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
X
Cookie Policy
ingentaconnect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more