Skip to main content

The In Vitro Effect of N-Acetylcysteine on Prothrombin Time in Plasma Samples From Healthy Subjects

Buy Article:

$43.00 plus tax (Refund Policy)

ACADEMIC EMERGENCY MEDICINE 2011; 18:351–354 © 2011 by the Society for Academic Emergency Medicine Abstract Objectives: 

In the treatment of acetaminophen toxicity, clinicians believe that N-acetylcysteine (NAC) artificially elevates prothrombin time (PT), potentially obscuring signs of liver damage. However, the effect of NAC on human blood coagulation remains unverified. The purpose of this study was to evaluate the effect of NAC on PT prolongation in human plasma. Methods: 

The authors obtained blood samples from 33 volunteer subjects. The blood plasma samples were divided into four 1-mL aliquots. The first aliquot was used as a control. To three additional aliquots, varying amounts of NAC were added, maintaining constant volume with a maximum dilution of 0.5%. The four concentrations of NAC (control, 250, 500, or 1,000 mg/L) were incubated at 37°C for 1 hour, and PT was measured. PT values were compared using fixed effects regression. Results: 

Mean (± standard deviation [SD]) PT values for the control, 100, 500, and 1,000 mg/L NAC values were 13.9 (±1.01), 14.2 (±1.08), 15.5 (±1.21), and 17.4 (±1.72) seconds, respectively. At the 1,000 mg/L concentration, two PTs exceeded 22 seconds, and half exceeded 17 seconds. PT increased with NAC concentrations (fixed effects regression p < 0.001) in a dose-dependent manner. Conclusions: 

In this in vitro human model, NAC had a dose-dependent effect on PT.
No References
No Citations
No Supplementary Data
No Data/Media
No Metrics

Document Type: Research Article

Publication date: 2011-04-01

  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more