Provider: Ingenta Connect
Database: Ingenta Connect
TY - ABST
AU - Vasiliou, Sylvia A.
AU - Ali, Fahad R.
AU - Haddley, Kate
AU - Cardoso, M. Cristina
AU - Bubb, Vivien J.
AU - Quinn, John P.
TI - The
JO - Addiction Biology
PY - 2012-01-01T00:00:00///
VL - 17
IS - 1
SP - 156
EP - 170
N2 - ABSTRACT
We demonstrated that the genotype of the variable number tandem repeats (VNTRs) in the linked polymorphic region (LPR) of the 5′ promoter and in the intron 2 (Stin2) transcriptional regulatory domains of the serotonin transporter
SLC6A4 gene determined its promoter interactions with transcription factors and co‐activators in response to cocaine in the JAr cell line. The LPR variants contain 14 (short, s) or 16 (long, l) copies of a 22–23 bp repeat element, whereas the Stin2 VNTR
exists as three variants containing 9, 10 or 12 copies of a 16–17 bp repeat. We observed a differential effect of cocaine on the association of the promoter with the transcription factor CTCF, which bound to both LPR alleles prior to cocaine exposure but only to the l‐allele
following exposure. Significantly, this differential effect of cocaine was correlated with the binding of the transcriptional regulator MeCP2 specifically to the s‐allele and recruiting the histone deacetylase complex (HDAC). Concurrently, cocaine increased the association of
positive histone marks over the SLC6A4 gene locus. At the Stin2 domain, we lost binding of the transcription factor YB‐1, while CTCF remained bound. Our biochemical data are consistent with differential reporter gene activity directed by the individual or dual domains in response
to cocaine in an Epstein–Barr virus‐based episome model of stable transfections. These observations suggest that exposure of JAr cells to cocaine may result in differential binding of transcription factors and activators based on a specific genotype that might alter epigenetic
parameters affecting gene expression after the initial challenge.
UR - http://www.ingentaconnect.com/content/bpl/adb/2012/00000017/00000001/art00015
M3 - doi:10.1111/j.1369-1600.2010.00288.x
UR - https://doi.org/10.1111/j.1369-1600.2010.00288.x