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Saturation Transfer Difference NMR Studies of the Interaction of the Proteinkinase CK2 with Peptides

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Abstract:

Saturation Transfer Difference NMR (STD NMR) is used for the detection of the binding constant of the decapeptide RRRDDDSDDD with CK2α, the catalytic subunit of the proteinkinase CK2. For this work a valid irradiation frequency of the CK2α had to be found ensuring that no peptide resonance is affected by the irradiation. This is the principle problem for investigations of protein peptide interactions by STD NMR due to the similarity of protein and peptide resonances. It is shown that by careful selection of the irradiation point a KD value averaging to 1 mM can be found. In addition, preferred binding sites of the peptide are detected and it is shown that the side chains of serine and aspartate are closest to the protein surface.

Keywords: KD Determination; protein-peptide interaction; proteinkinase CK2; saturation difference NMR

Document Type: Research Article

DOI: http://dx.doi.org/10.2174/092986612802084447

Publication date: September 1, 2012

More about this publication?
  • Protein & Peptide Letters publishes short papers in all important aspects of protein and peptide research, including structural studies, recombinant expression, function, synthesis, enzymology, immunology, molecular modeling, drug design etc. Manuscripts must have a significant element of novelty, timeliness and urgency that merit rapid publication. Reports of crystallisation, and preliminary structure determinations of biologically important proteins are acceptable. Purely theoretical papers are also acceptable provided they provide new insight into the principles of protein/peptide structure and function.
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