Antimicrobial Peptides from Adenanthera pavonina L. Seeds: Characterization and Antifungal Activity
Authors: Ribeiro Soares, Julia; de Oliveira Carvalho, Andre; Silva dos Santos, Izabela; Lima Tavares Machado, Olga; V. Nascimento, Viviane; Maria Vasconcelos, Ilka; Teixeira da Silva Ferreira, Andre; Enrique de Aguilar Perales, Jonas; Moreira Gomes, Valdirene
Source: Protein and Peptide Letters, Volume 19, Number 5, May 2012 , pp. 520-529(10)
Publisher: Bentham Science Publishers
Abstract:In this study, the antifungal activity of peptides extracted from Adenanthera pavonina seeds was assessed. Peptides were extracted and fractionated by DEAE-Sepharose chromatography. The non-retained D1 fraction efficiently inhibited the growth of the pathogenic fungi. This fraction was later further fractionated by reversed-phase chromatography, resulting in 23 sub-fractions. All separation processes were monitored by tricine-SDS-PAGE. Fractions H11 and H22 strongly inhibited the growth of Saccharomyces cerevisiae and Candida albicans. Fraction H11 caused 100% death in S. cerevisiae in an antimicrobial assay. The complete amino acid sequence of the peptide in fraction P2 was determined, revealing homology to plant defensins, which was named ApDef1. Peptides from fraction H22 were also sequenced.
Keywords: Adenanthera pavonina; Candida albicans; Saccharomyces cerevisiae; antimicrobial assay; defensin; fungicide activity; glycine-rich peptides; membrane permeabilization; pathogenic fungi; reversed-phase chromatography
Document Type: Research Article
Publication date: 2012-05-01
- Protein & Peptide Letters publishes short papers in all important aspects of protein and peptide research, including structural studies, recombinant expression, function, synthesis, enzymology, immunology, molecular modeling, drug design etc. Manuscripts must have a significant element of novelty, timeliness and urgency that merit rapid publication. Reports of crystallisation, and preliminary structure determinations of biologically important proteins are acceptable. Purely theoretical papers are also acceptable provided they provide new insight into the principles of protein/peptide structure and function.
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- In this Subject: Anatomy & Physiology
- By this author: Ribeiro Soares, Julia ; de Oliveira Carvalho, Andre ; Silva dos Santos, Izabela ; Lima Tavares Machado, Olga ; V. Nascimento, Viviane ; Maria Vasconcelos, Ilka ; Teixeira da Silva Ferreira, Andre ; Enrique de Aguilar Perales, Jonas ; Moreira Gomes, Valdirene