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5'-Methylthioadenosine Nucleosidase from Yellow Lupine (Lupinus luteus): Molecular Characterization and Mutational Analysis

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Abstract:

This is report of mutational analysis of higher plant 5'-methylthioadenosine nucleosidase (MTAN). We identified and characterized the gene encoding yellow lupine (Lupinus luteus) MTAN (LlMTAN). The role of active site amino acids residues Glu24, Phe134, Glu188 and Asp211 was analyzed by site-directed mutagenesis. The Glu24Gln and Asp211Asn substitutions completely abolished the enzyme activity. The Glu188Gln mutant showed only trace activity toward 5'-methylthioadenosine. These results indicate that these three amino acid residues are necessary for enzyme activity. Furthermore, as the result of replacement of Phe134 by less bulky leucine, LlMTAN acquired the ability to bind and hydrolyze S-adenosylhomocysteine. We also analyzed the sequence of the LlMTAN promoter region. It appeared that there may be a direct link between LlMTAN expression regulation and sulfate metabolism.





Keywords: 5'-Methylthioadenosine; 5'-Methylthioadenosine nucleosidase; His-Mag agarose beads; LlMTAN; Lupinus luteus; MTA cycle; Plant Cis-acting Regulatory DNA Elements; Site-directed mutagenesis; TALON resin; analogous; clones; exons; gene encoding; gene expression; lupine; sulfur-compounds; variants

Document Type: Research Article

DOI: http://dx.doi.org/10.2174/092986611795714023

Publication date: August 1, 2011

More about this publication?
  • Protein & Peptide Letters publishes short papers in all important aspects of protein and peptide research, including structural studies, recombinant expression, function, synthesis, enzymology, immunology, molecular modeling, drug design etc. Manuscripts must have a significant element of novelty, timeliness and urgency that merit rapid publication. Reports of crystallisation, and preliminary structure determinations of biologically important proteins are acceptable. Purely theoretical papers are also acceptable provided they provide new insight into the principles of protein/peptide structure and function.
ben/ppl/2011/00000018/00000008/art00008
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