Site-Directed Mutagenesis to Identify Key Residues in Structure-Function Relationship of Winged Bean Chymotrypsin-Trypsin Inhibitor and 3-D Structure Prediction
Keywords: 3-D Structure Prediction; Biosym Technologies; ETI; IPTG; LB-ampicillin agar; Molecular modeling; PCR; Plant proteinase inhibitors (PI); Site Directed Mutagenesis; WbCTI mutants; bacterial lysate; chromatography; reactive site loop; serine protease inhibitor; trypsin and chymotrypsin
Document Type: Research Article
Publication date: 2011-05-01
- Protein & Peptide Letters publishes short papers in all important aspects of protein and peptide research, including structural studies, recombinant expression, function, synthesis, enzymology, immunology, molecular modeling, drug design etc. Manuscripts must have a significant element of novelty, timeliness and urgency that merit rapid publication. Reports of crystallisation, and preliminary structure determinations of biologically important proteins are acceptable. Purely theoretical papers are also acceptable provided they provide new insight into the principles of protein/peptide structure and function.