Isolation and Detection of Proteins with Nano-Particles and Microchips for Analyzing Proteomes on a Large Scale Basis
Abstract:The advent of sugar-immobilized gold nano-particles (SGNPs), lipid-based nanoparticles, nanochromatography and nano-electrophoresis has revolutionized the methodology for proteins purification and proteomic research. This review provides an overview on the effective method developed for fast purification of proteins from extracts using SGNPs. In addition, the current application of microfluidic systems for analytical purposes in biochemistry will also be explored that include the micro total analysis systems (μ-TAS) and lab-on-a-chip (LOC) analyses which are capable of isolation and detection of proteins at the nanogram level. Finally, we describe why the lipid-based nano-particles (LBNPs) can enable the analysis in microchip electroseparation and how anionic and cationic LBNPs can be used for proteins separation.
Keywords: CE equipment; Chromatography; Microchips; Nanoparticles; Proteomes; TAS; capillary electrochromatography; lab-on-a-chip; lab-on-a-chip (LOC); lipid-based nano-particles (LBNPs); micro-total-analysis systems; microfluidic systems; nano-electrophoresis; nanochromatography; sugar-immobilized gold nano-particles (SGNPs)
Document Type: Research Article
Publication date: April 1, 2011
- Protein & Peptide Letters publishes short papers in all important aspects of protein and peptide research, including structural studies, recombinant expression, function, synthesis, enzymology, immunology, molecular modeling, drug design etc. Manuscripts must have a significant element of novelty, timeliness and urgency that merit rapid publication. Reports of crystallisation, and preliminary structure determinations of biologically important proteins are acceptable. Purely theoretical papers are also acceptable provided they provide new insight into the principles of protein/peptide structure and function.