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Purification and Partial Characterization of a New Pro-Inflammatory Lectin from Bauhinia bauhinioides Mart (Caesalpinoideae) Seeds

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Abstract:

A new galactose-specific lectin, named BBL, was purified from seeds of Bauhinia bauhinioides by precipitation with ammonium sulfate, followed by two steps of ion exchange chromatography. BBL haemagglutinated rabbit erythrocytes (native and treated with proteolytic enzymes) showing stability even after exposure to 60 °C for one hour. The lectin haemagglutinating activity was optimum between pH 8.0 and 9.0 and inhibited after incubation with D-galactose and its derivatives, especially α-methyl-D-galactopyranoside. The pure protein possessed a molecular mass of 31 kDa by SDS-PAGE and 28.310 Da by mass spectrometry. The lectin pro-inflammatory activity was also evaluated. The s.c. injection of BBL into rats induced a dose-dependent paw edema, an effect that occurred via carbohydrate site interaction and was significantly reduced by L-NAME, suggesting an important participation of nitric oxide in the late phase of the edema. These findings indicate that BBL can be used as a tool to better understand the mechanisms involved in inflammatory responses.





Keywords: -methyl-D-galactopyranoside; BBL; Electrospray ionization mass spectrometry (ESI-MS); HPLC; L-NAME; Lectin; Lectin Purification; Molecular Mass Determination; Rat Paw Edema Model; bauhinia bauhinioides; bovine serum albumin (BSA); caesalpinoideae; galactose-specific lectin; pro-inflammatory activity; proteolytic enzymes

Document Type: Research Article

DOI: http://dx.doi.org/10.2174/092986611794653987

Publication date: April 1, 2011

More about this publication?
  • Protein & Peptide Letters publishes short papers in all important aspects of protein and peptide research, including structural studies, recombinant expression, function, synthesis, enzymology, immunology, molecular modeling, drug design etc. Manuscripts must have a significant element of novelty, timeliness and urgency that merit rapid publication. Reports of crystallisation, and preliminary structure determinations of biologically important proteins are acceptable. Purely theoretical papers are also acceptable provided they provide new insight into the principles of protein/peptide structure and function.
ben/ppl/2011/00000018/00000004/art00009
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