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Myoglobin as a New Fluorescence Probe to Sense H2S

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Abstract:

A new, fast, simple and cost-effective sensing device for monitoring H2S has been developed. Proof-of-principle results showing that a commercial and cheap Myoglobin (Mb) can be successfully used as a biological probe for a fluorescence biosensor for H2S detection are reported. Two different commercial labels Cy3 and Atto620 were selected for this study. A high selectivity for detecting H2S against other thiols was found. The applicability of the proposed sensing system was successfully explored not only in solution but also when applied in the form of a solid state device.





Keywords: FRET; Fluorescence; Sol-gel; biosensors; heme-proteins

Document Type: Research Article

DOI: https://doi.org/10.2174/092986611794578378

Publication date: 2011-03-01

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  • Protein & Peptide Letters publishes short papers in all important aspects of protein and peptide research, including structural studies, recombinant expression, function, synthesis, enzymology, immunology, molecular modeling, drug design etc. Manuscripts must have a significant element of novelty, timeliness and urgency that merit rapid publication. Reports of crystallisation, and preliminary structure determinations of biologically important proteins are acceptable. Purely theoretical papers are also acceptable provided they provide new insight into the principles of protein/peptide structure and function.
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