Transient Expression of Chicken Antimicrobial Peptides by Mouse Mammary Carcinoma Cells C127
Keywords: Anti-rabbit immunoglobulin G; Antibacterial activity; Antimicrobial peptides; BCA Protein Assay Reagent kit; C127 cells; DNA sequencing; EDTA; Escherichia coli; Fetal bovine serum; Fowlicidin-3; Human β-defensin 2; Mouse Mammary Carcinoma Cells C127; Na3VO4; PMD19T vectors; Reverse Transcription Real Time-PCR Analysis; Staphylococcus. aureus; Triton X-100; UV- spectrometry; XhoI-cleaved pBC1; ampicillin; antimicrobial peptide; cDNA; cathelicidin-type antimicrobial peptides; cell lysis buffer; eukaryotic expression systems; fowlicidin-1; fowlicidin-3; gel electrophoresis; glyceraldehyde 3-phosphate dehydrogenase; gram-negative; gram-positive; horseradish; human cathelicidin LL-37; hydrocortisone; immunoprecipitation; insulin; leupeptin; pBC1; pBC1 vector; pBCFow-1; pBCFow-3; polymerase chain reaction; prolactin; real time (RT)-PCR; sodium dodecyl sulfate; sodium pyrophosphate; transgenic mice; transient expression; trichloroacetic acid method; vector; western blotting; β-casein promoter; β-glycerophosphate
Document Type: Research Article
Publication date: 2010-12-01
- Protein & Peptide Letters publishes short papers in all important aspects of protein and peptide research, including structural studies, recombinant expression, function, synthesis, enzymology, immunology, molecular modeling, drug design etc. Manuscripts must have a significant element of novelty, timeliness and urgency that merit rapid publication. Reports of crystallisation, and preliminary structure determinations of biologically important proteins are acceptable. Purely theoretical papers are also acceptable provided they provide new insight into the principles of protein/peptide structure and function.