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Transient Expression of Chicken Antimicrobial Peptides by Mouse Mammary Carcinoma Cells C127

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Abstract:

Fowlicidin-3 and fowlicidin-1 are cathelicidin-type antimicrobial peptides found in chicken. They effectively inhibit the proliferation of many gram-positive and gram-negative bacteria. To obtain sufficient amounts of these peptides for possible use in therapeutic applications, DNA encoding each full-length gene, including all exons and introns, was fused to the β-casein promoter in a pBC1 vector that was then introduced into C127 cells. The full-length precursor proteins were expressed in response to a mixture of insulin, hydrocortisone, and prolactin. Processed fowlicidin-1 and fowlicidin- 3, as well as their precursors, were found in the cell culture media, which suggested that they could be processed and secreted. These transgenic peptides had antibacterial activity. Thus, transfected C127 cells may serve as an in vitro transgenic cell system that can be used to evaluate if specific gene constructs can be efficiently expressed in the mammary glands of transgenic mice.





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  • Protein & Peptide Letters publishes short papers in all important aspects of protein and peptide research, including structural studies, recombinant expression, function, synthesis, enzymology, immunology, molecular modeling, drug design etc. Manuscripts must have a significant element of novelty, timeliness and urgency that merit rapid publication. Reports of crystallisation, and preliminary structure determinations of biologically important proteins are acceptable. Purely theoretical papers are also acceptable provided they provide new insight into the principles of protein/peptide structure and function.
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