Quantitative Effects of Magnesium Chloride Stress on Aggregation of Sup35p in [psi-] Yeast Cells
Keywords: Confocal Microscopy; FRAP assay; Green Fluorescent Protein; Immo-bilon polyvinylidene difluoride sheets; Immunoblotting; Live-Time Fluorescence Analysis; Magnesium chloride; NGMC diffusion; PSI+; SDD-AGE; SDS-PAGE; SDS-insoluble Sup35p; SDS-resistant Sup35p polymers; Saccharomyces cerevisiae; Sup35p; Sup35p in [psi-] Yeast Cells; Tris-borate-EDT; Western Blot Analysis; [PSI+]; adenine; anti-Sup35p antiserum; fluorescence recovery after photobleaching; guanidine curing; guanidine-hydrochloride; isogenic [Gpsi-] cells; nonsense suppression; prion; quantitative effects; semi-denaturing detergent-agarose gel electrophoresis; stress
Document Type: Research Article
Publication date: 01 December 2010
- Protein & Peptide Letters publishes short papers in all important aspects of protein and peptide research, including structural studies, recombinant expression, function, synthesis, enzymology, immunology, molecular modeling, drug design etc. Manuscripts must have a significant element of novelty, timeliness and urgency that merit rapid publication. Reports of crystallisation, and preliminary structure determinations of biologically important proteins are acceptable. Purely theoretical papers are also acceptable provided they provide new insight into the principles of protein/peptide structure and function.