Purification and Kinetics of Bovine Kidney Cortex Glutathione Reductase
Abstract:Glutathione reductase was purified 34806-fold with a final yield of 85 % from the bovine kidney cortex. Some molecular and kinetic properties of purified enzyme are investigated. Product inhibition studies showed that the enzyme obeys ‘branched’ mechanism: KmNADPH 18 ± 3 μM and KmGSSG 65 ± 5 μM were determined.
Document Type: Research Article
Publication date: 2010-05-01
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