Characterization of a New Defensin from Cowpea (Vigna unguiculata (L.) Walp.)

Authors: Padovan, Lara; Segat, Ludovica; Tossi, Alessandro; Calsa Jr., Tercilio; Ederson, Akio K.; Brandao, Lucas; Guimaraes, Rafael L.; Pandolfi, Valesca; Pestana-Calsa, Maria Clara; Belarmino, Luis Carlos; Benko-Iseppon, Ana Maria; Crovella, Sergio

Source: Protein and Peptide Letters, Volume 17, Number 3, March 2010 , pp. 297-304(8)

Publisher: Bentham Science Publishers

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Abstract:

Using Phaseoleae defensins available in databases, a putative defensin gene was isolated in cowpea (Vigna unguiculata (L.) Walp.) and cloned from genomic cowpea DNA. The putative mature defensin sequence displays the characteristic defensins residues arrangement, secondary and tertiary structures were predicted and splicing analysis was performed. Using RT-PCR, defensin expression and differences in response to biotic stimuli between infected and non infected plants were tested.





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  • Protein & Peptide Letters publishes short papers in all important aspects of protein and peptide research, including structural studies, recombinant expression, function, synthesis, enzymology, immunology, molecular modeling, drug design etc. Manuscripts must have a significant element of novelty, timeliness and urgency that merit rapid publication. Reports of crystallisation, and preliminary structure determinations of biologically important proteins are acceptable. Purely theoretical papers are also acceptable provided they provide new insight into the principles of protein/peptide structure and function.

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