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Efficient Manual Fmoc Solid-Phase Synthesis of the N-Terminal Segment of Surfactant Protein B (SP-B1-25)

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Abstract:

The N-terminal 25 residue segment of human surfactant protein B (SP-B1-25) was synthesised in 26% yield by manual Fmoc solid-phase peptide synthesis (Fmoc SPPS) using low-loading Fmoc-Gly-Wang resin. Substantial oxidation of Met21 occurred during the synthesis, and the addition of Bu4NBr to a TFA/water/EDT/TIS cleavage cocktail enabled facile reduction of Met(O)21-SP-B1-25 to SP-B1-25. The methods described herein are generally applicable to the Fmoc SPPS of difficult sequences containing methionine.





Keywords: Fmoc solid-phase peptide synthesis; Surfactant protein B (SP-B); low-loading resin; methionine sulfoxide; peptide aggregation; tetrabutylammonium bromide

Document Type: Research Article

DOI: https://doi.org/10.2174/092986609788681706

Publication date: 2009-07-01

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  • Protein & Peptide Letters publishes short papers in all important aspects of protein and peptide research, including structural studies, recombinant expression, function, synthesis, enzymology, immunology, molecular modeling, drug design etc. Manuscripts must have a significant element of novelty, timeliness and urgency that merit rapid publication. Reports of crystallisation, and preliminary structure determinations of biologically important proteins are acceptable. Purely theoretical papers are also acceptable provided they provide new insight into the principles of protein/peptide structure and function.
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