Efficient Production of Human β-Defensin 2 (HBD2) in Escherichia coli

Authors: Vargues, Thomas; Morrison, Gareth J.; Seo, Emily S.; Clarke, David J.; Fielder, Helen L.; Bennani, Julien; Pathania, Uday; Kilanowski, Fiona; Dorin, Julia R.; Govan, John R.W.; Mackay, C. L.; Uhrin, Dusan; Campopiano, Dominic J.

Source: Protein and Peptide Letters, Volume 16, Number 6, June 2009 , pp. 668-676(9)

Publisher: Bentham Science Publishers

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Abstract:

Human β-defensin 2 (HBD2) has been shown to interact with pathogenic bacteria and components of the mammalian innate and adaptive immune response. We describe a quick and reliable method for the production of HBD2 in Escherichia coli. HBD2 was expressed as an insoluble fusion, chemically cleaved and oxidised to give a single, folded HBD2 β-isoform. The purified peptide was analysed by high resolution mass spectrometry, displayed a well-dispersed ¹H NMR spectrum, was a chemoattractant to HEK293 cells expressing CCR6 and acted as an antimicrobial agent against E. coli, P. aeruginosa, C. albicans and S. aureus.

Keywords: HBD2; Defensin; Peptide; E. coli; Chemotaxis; Antimicrobial activity

Document Type: Research article

Publication date: 2009-06-01

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Protein & Peptide Letters publishes short papers in all important aspects of protein and peptide research, including structural studies, recombinant expression, function, synthesis, enzymology, immunology, molecular modeling, drug design etc. Manuscripts must have a significant element of novelty, timeliness and urgency that merit rapid publication. Reports of crystallisation, and preliminary structure determinations of biologically important proteins are acceptable. Purely theoretical papers are also acceptable provided they provide new insight into the principles of protein/peptide structure and function.