Estimation of Affinity of HLA-A*0201 Restricted CTL Epitope Based on the SCORE Function

Authors: Zhi-Hua, Lin; Huai-Liang, Wang; Bo, Zhu; Yuan-Qiang, Wang; Yong, Lin; Yu-Zhang, Wu

Source: Protein and Peptide Letters, Volume 16, Number 5, May 2009 , pp. 561-569(9)

Publisher: Bentham Science Publishers

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Abstract:

A set of 70 peptides with affinity for the class I MHC HLA-A*0201 molecule was subjected to quantitative analyses of structure-affinity relationship based on the SCORE function with good results (r2=0.6982, q2=0.6188, RMS=0.280). Then the `leave-one-out' cross-validation (LOO-CV) and an outer test set including 18 outer samples were used to validate the QSAR model, and the results show that the QSAR model has good predictability for outside samples. Statistical analysis showed that the hydrophobic and hydrogen bond interaction played a significant role in peptide-MHC molecule binding. The study also provided useful information for structure modification of CTL epitope, and laid theoretical base for molecular design of therapeutic vaccine.

Keywords: SCORE function; HLA-A*0201; CTL epitope; Affinity; QSAR

Document Type: Research article

DOI: http://dx.doi.org/10.2174/092986609788167815

Publication date: 2009-05-01

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Protein & Peptide Letters publishes short papers in all important aspects of protein and peptide research, including structural studies, recombinant expression, function, synthesis, enzymology, immunology, molecular modeling, drug design etc. Manuscripts must have a significant element of novelty, timeliness and urgency that merit rapid publication. Reports of crystallisation, and preliminary structure determinations of biologically important proteins are acceptable. Purely theoretical papers are also acceptable provided they provide new insight into the principles of protein/peptide structure and function.