Purification and Characterization of Human Brain Serine Racemase Expressed in Moderately Halophilic Bacteria
We have successfully expressed an active human brain serine racemase (hSR) with His-tag using moderate halophile. The purified His-hSR showed high elimination and racemization activities on L-serine: the elimination activity was 2.6-fold higher than racemization activity. Both enzyme activities showed an optimum reaction pH at around 9.0 and were stimulated 5- to 7-fold by such divalent cations as Mg++, Mn++ and Ca++.
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Document Type: Research Article
Publication date: 01 February 2009
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