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Aptabodies - New Type of Artificial Receptors for Detection Proteins

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Abstract:

We report on a new type of artificial receptor formed by hybridization of two DNA aptamers for human thrombin (aptabody). This aptasensor based on multiwalled carbon nanotubes allowed us to detect thrombin with detection limit of 0.3 nM, which was 3 times better in comparison with conventional aptamer.





Keywords: Biosensor; DNA aptamers; Electrochemical quartz crystal microbalance; Human thrombin; Hybridization; Multiwalled carbon nanotubes

Document Type: Research Article

DOI: https://doi.org/10.2174/092986608785203656

Publication date: 2008-08-01

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  • Protein & Peptide Letters publishes short papers in all important aspects of protein and peptide research, including structural studies, recombinant expression, function, synthesis, enzymology, immunology, molecular modeling, drug design etc. Manuscripts must have a significant element of novelty, timeliness and urgency that merit rapid publication. Reports of crystallisation, and preliminary structure determinations of biologically important proteins are acceptable. Purely theoretical papers are also acceptable provided they provide new insight into the principles of protein/peptide structure and function.
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