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Propionate Sensor Using Coenzyme-A Transferase and Acyl-CoA Oxidase

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We developed an amperometric propionate sensor using comprised of two recombinant enzymes, propionate coenzyme A CoA transferase from Clostridium propionicum and short-chain acyl-CoA oxidase from Arabidopsis thaliana. Response current increased linearly with increase in propionate concentration from 10 μM to 100 μM. The detection limit was 10 μM propionate.

Keywords: Propionate; amperometry; enzyme sensor; propionate CoA transferase; short-chain acyl-CoA oxidase; volatile fatty acid

Document Type: Research Article


Publication date: 2008-08-01

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  • Protein & Peptide Letters publishes short papers in all important aspects of protein and peptide research, including structural studies, recombinant expression, function, synthesis, enzymology, immunology, molecular modeling, drug design etc. Manuscripts must have a significant element of novelty, timeliness and urgency that merit rapid publication. Reports of crystallisation, and preliminary structure determinations of biologically important proteins are acceptable. Purely theoretical papers are also acceptable provided they provide new insight into the principles of protein/peptide structure and function.
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