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Effects of Entomopathogenic Bacterium Photorhabdus temperata Infection on the Digestive Enzymes of Diatraea saccharalis (Lepidoptera: Crambidae) Larvae

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This study investigated the effects of Photorhabdus temperata infection on the activities of digestive enzymes of the sugarcane stalk borer Diatraea saccharalis. Non-infected D. saccharalis larvae present a major α-amylase, several proteinases, three sucrose hydrolases and two α-glucosidases in their midgut. Analysis of these hydrolases by electrophoresis and “in gel” assays showed that the activities of all enzymes decreased following infection, with an initial decline observed 12 h after infection. The activities of α-glucosidases decreased by 50% twelve hours after infection, whereas, at this time, the α-galactosidase activities decreased by 70%. Interestingly, the animals died 48 h after infection, but approximately 5% of all the enzymes tested remained active in the midgut following host death. At this time, most of the cultivable native intestinal bacteria had died.

Keywords: Diatraea saccharalis; Photorhabdus temperate; infection; insect digestion; intestinal microbiota

Document Type: Research Article

DOI: http://dx.doi.org/10.2174/092986608785133618

Publication date: July 1, 2008

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  • Protein & Peptide Letters publishes short papers in all important aspects of protein and peptide research, including structural studies, recombinant expression, function, synthesis, enzymology, immunology, molecular modeling, drug design etc. Manuscripts must have a significant element of novelty, timeliness and urgency that merit rapid publication. Reports of crystallisation, and preliminary structure determinations of biologically important proteins are acceptable. Purely theoretical papers are also acceptable provided they provide new insight into the principles of protein/peptide structure and function.
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