Preparation of a Tat-Related Transporter Peptide for Carrying the Adenovirus Vector into Cells
Authors: Kawasaki, Koichi; Kida, Shinya; Eto, Yusuke; Maeda, Mitsuko; Yoshioka, Yasuo; Nakagawa, Shinsaku; Hojo, Keiko; Tsuda, Yuko; Mayumi, Tadanori
Source: Protein and Peptide Letters, Volume 15, Number 2, February 2008 , pp. 219-222(4)
Publisher: Bentham Science Publishers
Abstract:We synthesized a Tat-related peptide acetyl-Gly-Arg-Lys-Lys-Arg-Arg-Gln-Arg-Arg-Arg-Pro-Pro-Gln-Gly- Cys amide, Ac-Tat48-60-Gly-Cys-NH2, having high intracellular permeability, and conjugated this peptide to adenovirus vector to enhance gene transfer efficiency of adenovirus vector into cells. The peptide was prepared by the solid-phase peptide synthesis method and a bifunctional crosslinker 6-maleimidohexanoic acid N-hydroxysuccinimide ester was used to conjugate the peptide to adenovirus vector containing luciferase gene. The novel conjugate of adenovirus vector and Ac-Tat48-60-Gly-Cys-NH2 peptide exhibited excellent gene transfer efficacy in B16BL6 cells.
Document Type: Research Article
Publication date: 2008-02-01
- Protein & Peptide Letters publishes short papers in all important aspects of protein and peptide research, including structural studies, recombinant expression, function, synthesis, enzymology, immunology, molecular modeling, drug design etc. Manuscripts must have a significant element of novelty, timeliness and urgency that merit rapid publication. Reports of crystallisation, and preliminary structure determinations of biologically important proteins are acceptable. Purely theoretical papers are also acceptable provided they provide new insight into the principles of protein/peptide structure and function.