Skip to main content

Cloning, Expression, Purification, Characterization, Crystallization and X-Ray Diffraction of Bifunctional Pyrimidine Deaminase/Reductase from Shigella flexneri 2a

Buy Article:

$63.00 plus tax (Refund Policy)

Abstract:

Bifunctional pyrimidine deaminase/reductase (RibD) plays an important role during riboflavin biosynthesis in many microorganisms. The 40.4 kDa RibD from Shigella flexneri 2a has been cloned, expressed, purified and characterized. Three Crystals of RibD have been obtained by the hanging-drop technique at 291 K using PEG 20k or NaCl as precipitant. The RibD crystal using PEG 20k as precipitant diffracted to 2.5Å.





Keywords: RibD; Shigella flexneri 2a; bifunctional pyrimidine deaminase/reductase; crystallization; expression; riboflavin biosythesis

Document Type: Research Article

DOI: http://dx.doi.org/10.2174/092986607782110347

Publication date: September 1, 2007

More about this publication?
  • Protein & Peptide Letters publishes short papers in all important aspects of protein and peptide research, including structural studies, recombinant expression, function, synthesis, enzymology, immunology, molecular modeling, drug design etc. Manuscripts must have a significant element of novelty, timeliness and urgency that merit rapid publication. Reports of crystallisation, and preliminary structure determinations of biologically important proteins are acceptable. Purely theoretical papers are also acceptable provided they provide new insight into the principles of protein/peptide structure and function.

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more