Crystallization and Preliminary Crystallographic Study of DNA Polymerase from Pyrococcus furiosus
Abstract:A new member of archaeal DNA polymerase from Pyrococcus furiosus was crystallized. Diffraction data to 3.1 Å of the selenomethionine-derivatized crystal were collected, and preliminary crystallographic study has been completed. The crystal belongs to the space group C2 with unit cell parameters of a = 93.2 Å, b = 124.9 Å, c = 87.7 Å, α = 90 , β = 109.7® , and γ = 90 . Assuming the presence of one molecule in the asymmetric unit, the solvent content of the crystal is estimated to be 54%, corresponding to a Matthews coefficient VM of 2.7Å3 Da-1.
Document Type: Research Article
Affiliations: Open Laboratory of Advanced Bioscience and Biotechnology (OLABB), Institute for Protein Research, Osaka University. 6-2-3 Furuedai, Suita, Osaka 565-0874, Japan.
Publication date: April 1, 2007
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