Provider: Ingenta Connect Database: Ingenta Connect Content: application/x-research-info-systems TY - ABST AU - Agueci, Francesco AU - Polticelli, Fabio AU - Sinibaldi, Federica AU - Cristina Piro, Maria AU - Santucci, Roberto AU - Fiorucci, Laura TI - Probing the Effect of Mutations on Cytochrome c Stability JO - Protein and Peptide Letters PY - 2007-04-01T00:00:00/// VL - 14 IS - 4 SP - 335 EP - 339 KW - circular dichroism KW - mutagenesis KW - guanidinium chloride unfolding KW - protein stability KW - Cytochrome c N2 - Although the tertiary structures of mitochondrial cytochromes c (cyts c) seem to be remarkably similar, there are variations in their amino acid sequences, stability and functional properties. GdnHCl-induced unfolding experiments on engineered yeast and horse cyt c were carried out with the aim to to clarify, at molecular level, some aspects concerning the stability of this class of proteins. The results obtained are discussed in the light of the three-dimensional structures of the two proteins.





UR - https://www.ingentaconnect.com/content/ben/ppl/2007/00000014/00000004/art00005 M3 - doi:10.2174/092986607780363989 UR - https://doi.org/10.2174/092986607780363989 ER -