Reevaluating the Capability of Taq DNA Polymerase: Long PCR Amplification

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Abstract:

We tested the ability of Taq DNA polymerase (Taq) to amplify long DNA fragments and showed that, if the conditions were set properly, Taq could successfully perform the “long PCR” up to 24 kb. The conditions include: (1) longer primers, (2) a 2-step cycling, and (3) a “long buffer.” We propose that the most important requirements are the survival rate of Taq at high temperatures and that of the primers against the 5' to 3' exonuclease activity of Taq.





Keywords: Taq DNA polymerase; long amplicons; primers; reaction buffer

Document Type: Research Article

DOI: http://dx.doi.org/10.2174/092986607780363934

Affiliations: Department of Chemistry and 2Recombinant Protein Expression Center (RPEC), Sejong University,98 Gunja-Dong, Gwangjin-Gu, Seoul, Korea, 143-747.

Publication date: April 1, 2007

More about this publication?
  • Protein & Peptide Letters publishes short papers in all important aspects of protein and peptide research, including structural studies, recombinant expression, function, synthesis, enzymology, immunology, molecular modeling, drug design etc. Manuscripts must have a significant element of novelty, timeliness and urgency that merit rapid publication. Reports of crystallisation, and preliminary structure determinations of biologically important proteins are acceptable. Purely theoretical papers are also acceptable provided they provide new insight into the principles of protein/peptide structure and function.
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