Skip to main content

Reevaluating the Capability of Taq DNA Polymerase: Long PCR Amplification

Buy Article:

$63.00 plus tax (Refund Policy)

We tested the ability of Taq DNA polymerase (Taq) to amplify long DNA fragments and showed that, if the conditions were set properly, Taq could successfully perform the “long PCR” up to 24 kb. The conditions include: (1) longer primers, (2) a 2-step cycling, and (3) a “long buffer.” We propose that the most important requirements are the survival rate of Taq at high temperatures and that of the primers against the 5' to 3' exonuclease activity of Taq.

No References
No Citations
No Supplementary Data
No Article Media
No Metrics

Keywords: Taq DNA polymerase; long amplicons; primers; reaction buffer

Document Type: Research Article

Affiliations: Department of Chemistry and 2Recombinant Protein Expression Center (RPEC), Sejong University,98 Gunja-Dong, Gwangjin-Gu, Seoul, Korea, 143-747.

Publication date: 01 April 2007

More about this publication?
  • Protein & Peptide Letters publishes short papers in all important aspects of protein and peptide research, including structural studies, recombinant expression, function, synthesis, enzymology, immunology, molecular modeling, drug design etc. Manuscripts must have a significant element of novelty, timeliness and urgency that merit rapid publication. Reports of crystallisation, and preliminary structure determinations of biologically important proteins are acceptable. Purely theoretical papers are also acceptable provided they provide new insight into the principles of protein/peptide structure and function.
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more